Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 81)

Section 6 Data Analysis Module
- 72 -
Fig. 6.30. The Allelic Data Spreadsheet.
• In Automatic Call mode, the iQ5 software creates the assignments based on the positions
of the vertical and horizontal bars
• In Manual Call mode, the Call column becomes editable through a menu. This can be
very useful if you want to change the definition of a sample from an unknown to a
positive control, for example
• The data in the spreadsheet may be copied to the clipboard for export to another
program by clicking in the top left corner of the spreadsheet and typing CTRL+C
6.7.4 Automatic/Manual Call
Select the type of analysis by clicking Automatic Call or Manual Call (Figure 6.31).
Fig. 6.31. The Automatic and Manual Call Options.
Automatic Call is the default option.
•
Choose Automatic Call for the software to make genotype assignments for every
unknown based on the positions of the vertical and horizontal bars and presents those
assignments in the data spreadsheet. If at least three positive (homozygous) controls are
specified for each fluorophore, the positions of the bars are based on the mean and
standard deviation of the threshold cycles or RFU values of the controls. If insufficient
numbers of controls are specified, then the position of each bar is determined by the
range of threshold cycles or RFU values in the selected fluorophores. The positions of the
bars may be manually adjusted by dragging them. Click Recalculate after any change in
the position of the vertical and horizontal bars and the software will make new genotype
assignments based on the adjusted positions.
• Choose Manual Call to make the genotype assignments directly into the data spreadsheet
or on the plot. You can use the manual call feature to change the definitions of one or