Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 30)

Section 4 Workshop Module
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Fig. 4.2. The Selected Plate Setup Area.
4.1.4 Selected Data File Area
Located in the top right of the Setup window, the Selected Data File area displays the selected
data file or the run set file name that you selected in the file browser area. The Notes box
displays the notes associated with either the run set or data file. Buttons in this area include:
•
Run: Used to initiate a real-time PCR experimental run.
• Begin End Point: Used to initiate an End Point run.
•
Analyze: Used to open a data file. You can also open the data file by double-clicking
on the data file name in the file browser area.
4.2 Plate Setup
The MyiQ2, MyiQ, and iQ5 Real-Time PCR Detection systems only display and analyze data from
wells defined in plate setup as containing sample and at least one fluorophore. In the Plate Setup
Editor window, you specify the type of sample and the fluorophores present in each well.
The Plate Setup Editor window is accessed from the main Workshop module window by clicking
on either Edit or Create New in the Selected Plate Setup window (see Figure 4.1), or by double-
clicking on an existing plate setup file from the file browser folder tree.
4.2.1 Plate Setup Editor Window
The Plate Setup Editor window (Figure 4.3) is comprised of a 96-well plate layout, functions for
specifying the sample type and fluorophores in each well, and a spreadsheet displaying the
definition in each fluorophore for any individual well.