Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 32)

Section 4 Workshop Module
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Defines Positive Controls
Paint Bucket icon: When the Paint Bucket icon is active and you click a well in the
plate setup, all wells in that replicate group will be assigned the currently selected
fluorophore.
Delete Fluorophore icon: When the Delete Fluorophore icon is active and you click in
a well in the plate setup, the currently selected fluorophore is removed from all wells in
that replicate group. It does not change the definition of the sample type in that well.
Delete All icon: When the Delete All icon is active and you click a well, all information
from that well is removed. The other members of a replicate group are not affected.
4.2.3 Editing or Creating a New Plate Setup
1. Open the Plate Setup Editor Window within the Workshop module by using one of the
following methods:
•
Click Create New in the plate setup display pane to enter the Plate Setup Editor
or
•
Click Plate. Select the desired plate setup file from the file tree directory. Double-click
the file name to go directly to the Plate Setup Editor
or
•
Click Plate. Select the desired plate setup file from the file tree directory. Click the file
name to open the plate setup in the bottom right section of the Workshop window.
Click Edit to open the plate setup in the Plate Setup Editor
or
• Click Data File. Select the desired data setup file from the file tree directory. Click the
file name to open the plate setup associated with the data file in the bottom right
section of the Workshop window. Click Edit to open the plate setup in the Plate Setup
Editor.
2. Enter or edit any notes about the plate setup in the Notes box.
3. Enter or edit the sample volume, seal type, and vessel type.
4. Enter or edit a name for the experiment.
5. Click Select/Add Fluorophores, and select or edit the fluorophores to be used on the
plate.
6. For most experiments leave the Whole Plate Loading box checked.
• With Whole Plate Loading, changes made to any fluorophore within a well are
extended to the other fluorophores within the well and within the replicate group.
• When Whole Plate Loading is deselected, no assumption is made about the sample
type in the second and subsequent fluorophores. The sample type can vary from
fluorophore to fluorophore and if there are standards you can define individual
dilution series for individual fluorophores.
7. Click a sample type icon.
8. Select the type of replicate loading desired.