Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 92)

Section 6 Data Analysis Module
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1. In the PCR Quant screen, assess threshold and baseline for the data file and then make
changes if necessary.
2. Click the Gene Expr tab.
3. Highlight and select the wells to be edited in the gene expression plate interface.
NOTE: Expanding the gene expression plate interface view by clicking on the “+” button
makes the entire plate area visible for easier well identification and selection.
4. Change the gene and condition assignments in these wells by typing in the gene and
condition pull-down menus, then click Enter to apply the name to the selected wells.
Entered names will then be added to the pull-down menu, and become available for
selection from this menu.
NOTE: The Gene and Condition Names have a character entry limit of 15 characters.
In Figure 6.39, wells B4, C4, D4 are selected, and are being changed from condition
name 1 Hr to 1.5 Hrs. The new condition name, 1.5 Hrs, was typed in the Condition
Name box.
In addition to the gene expression plate interface, gene and condition names can also be edited
post-run using Edit Plate. Opening this window when analyzing collected data by clicking on the
Edit Plate tab at the top of the screen will open a modified version of this window. In this
modified window you can edit sample type, replicates, standard concentrations and
primer/probe-gene names. For further details on post-run edit plate functionality, refer to section
6.11.
Fig. 6.39. Editing Gene and Condition Names in Gene Expression Plate Interface.
Copying Conditions to All Data Sets
The Copy conditions to all data sets button should be activated if you want all fluorophores
or data sets to have the same Condition Name(s) assigned to selected groups of wells. This is a
useful function if: