Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 24)

Section 2 Quick Guides
- 15 -
•
Minimize the Gene Expression Plate interface by clicking on the “–” button to return
to the standard view of the Gene Expr tab window
4. Click the Settings tab, then select Gene List to set your desired reference gene(s).
5. Click Normalized Expression (ddCt).
6. Click Recalculate to see your results.
7. Normalized expression results are graphed. The Data Table spreadsheet is accessed by
clicking Data Table. The Data Table spreadsheet lists the Condition and Gene name,
calculated expression values and C
T
values. Right-click to print or export this data to
Excel.
NOTE: Use the Settings tab, then select Gene List to enter a specific user-defined
gene reaction efficiency. Use the Settings tab, then select Condition List to select a
particular sample as a control sample.
If you need to compare these data to results obtained in other .opd files, you will need to enable
this file for Multi-file Gene Expression analysis, also called a Gene Study. To enable your file for
Gene Study:
1. Click the Enable for Gene Study button.
2. Go to the File menu to save your file.
Gene Study (Multiple File Gene Expression) Quick Guide
To create a Gene Study (for analysis of multiple .opd files):
1. For each .opd file you wish to combine to a Gene Study.
a. In the PCR Quant screen (with a .opd data file open), assess Threshold and
Baseline information for the data file and make changes if necessary.
b. Click the Gene Expr tab.
c. Make sure all files to be included in the Gene Study have the Enable for Gene
Study button active.
d. Save each individual file.
2. From the menu toolbar select File/New/Gene Study.
3. In the Gene Expression Study Manager select Add .opd’s.
4. Select the files that you wish to add to the Gene Study. More than one file can be
selected.
5. Once the files are added to the Gene Expression Study Manger, select OK.
6. Make any changes to Gene and Condition (for example, Sample and Treatment)
assignments in the Gene Expression Plate interface.
•
Expand the Gene Expression Plate interface view by clicking on the “+” button to
make well identification and selection easier. Highlight the wells in the gene
expression plate interface you wish to edit
•
Change the gene assignments in these wells by typing your desired name into the
gene pull-down men, then click enter to apply the name to the selected wells