Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 48)

Section 4 Workshop Module
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4.5.1 Selecting a Data File
1. Click the Data File button.
2. Use the file browser below the Data File button to locate the folder that contains the data
file, and then click on the file name to select it.
3. The protocol and plate setup used in the data file will appear in the Selected Protocol and
Selected Plate Setup windows. The plate setup that is shown can be either the Original
or Current (last saved) plate setup. If the data file protocol and plate setup are being
used as the conditions for running a new experiment, then the plate setup that is shown
in Selected Plate Setup window will be the one that is used to run the new experiment.
4.5.2 Opening a Data File
To open an optical data file from an amplification or melt curve experiment, select the name of
the data file in the file browser and click Analyze (or double-click the file name).
NOTE: Data files collected using an iQ5 Real-Time PCR Detection System can be opened when
the computer running iQ5 software (version 2.1) is connected to a MyiQ2 or MyiQ Real-Time
Detection System. A warning message will display at the bottom of the screen indicating that
while the data can be viewed and analyzed, the plate setup contains fluorophores that cannot be
used to collect new data using this plate setup. Similarly, opening a data file collected using a
MyiQ2 system while a MyiQ system is connected to the software will result in the same message
(Figure 4.18).
Fig. 4.18. Invalid Data File Error.
4.5.3 Opening a Pure Dye Calibration file
Opening a pure dye calibration file will not only show the pure dye fluorescent data in the PCR
Quant screen but will also write this calibration file to the appropriate calibration folder. This will
overwrite a calibration file of the same reaction vessel, seal, optical camera and thermal cycler,
and all subsequent experimental data will be collected and analyzed with the new calibration file.
4.5.4 Applying Alternate Pure Dye Calibrations (Alternate RME)
Optical data is saved to the data file together with the appropriate calibration files. For the MyiQ2
and iQ5 Real-Time systems, it is possible to overwrite the pure dye calibration files stored within
the .opd data file. This feature protects you from losing valuable experimental data if, for
example, the calibrations were conducted incorrectly or a run was completed with expired well
factors.
To apply an alternate pure dye calibration RME file to a data file;
1. Select the data file in the file browser.
2. Select Apply Alternate RMEs from the Tools menu.
3. Use the Select RME File Name dialog box to navigate to, then open the RME calibration
file.