Bio-Rad iQ™5 Optical System Software, Version 2.1 User Manual

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Section 6 Data Analysis Module

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The alternative to viewing the data in All Candidates mode is to view the data in Single Point
mode.

Adjust Graph

Rescale or change the amplification plot from linear to log, or vice versa, by selecting Adjust
Graph. The Chart Axes Range Definition window appears (Figure 6.19).

Fig. 6.19. The Chart Axes Range Definition Window.

Enter the maximum and minimum values for the x-and y-axes into the Y Max, Y Min, X Min, and
X Max scroll box(es), or use the up and down arrows in each scroll box.

Change the display to a semi-logarithmic view by clicking Log View. Click Log View again to
revert to a linear plot.

Revert the plot settings to the default by clicking Restore Auto Scaled Chart.

NOTE: You can also change the display to a semi-logarithmic view by selecting Log View in the
PCR Quant tab.

Define Trace Style Option

You can customize the display by selecting Define Trace Style option. The Define Trace Style
window appears (Figure 6.20).

Change the trace color and symbol, which are used to display data points, on a well-by-well basis
or in groups of sample types. You can preview all changes before you apply them by clicking
Preview.

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