Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 122)

Section 7 Calibrating the Instrument
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Calibrations are performed in the order as they appear in the tabs of the Calibration module:
• Mask Alignment
• Background Calibration
• Persistent Well Factor Generation
• Pure Dye Calibration
7.4.1 Performing Mask Alignment
Before you align the mask, complete the following steps:
1. Prepare an external well factor plate (see section 7.3)
2. Place the external well factor plate in the iCycler thermal cycler.
3. Select the Mask tab in the Calibration module to open the image window (Figure 7.1).
Fig. 7.1. The Mask Image Window for the MyiQ2 Real-Time PCR Detection System.
The main use of the Mask Image window is to adjust the masks although there are other uses
including capturing an image of the experimental plate to check the response of a probe, or to
assess the completion of a reaction. With a plate image you can obtain fluorescence readings for
each of the 96 individual wells.
To align the mask:
1. Click Home.
2. Click the Filter Position 2 option button.