Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 49)

Section 4 Workshop Module
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4. After the RME calibration file has been opened a Save Optical Data File dialog box will
appear. The existing pure dye calibration will be overwritten.
NOTE: It is recommended to save the optical data file with a different name as this will ensure
that the original file, with the original calibration data, is always maintained for reference.
4.5.5 Applying Alternate Background or Well Factors
If well factors have been collected incorrectly, or data has been collected with expired well
factors, you may apply an alternate well factor calibration to a data file.
When selecting an alternate well factors file to apply use Persistent Well Factor files only.
To apply an alternate Well Factor file to a dataset:
1. Select the data file in the file browser.
2. Select Apply Alternate Well Factors from the Tools menu.
3. Use the Select Well Factors File Name dialog box to navigate to, then open the desired
Well Factors calibration file
4. After the Well Factors calibration file has been opened, a Save Optical Data File dialog
box will appear. The existing well factor calibration for this data file will be overwritten.
The same approach can be used to apply alternate Background Factors.
NOTE: It is recommended to save the optical data file with a different name from the original to
ensure that the original file, with the original well factor calibration data, is maintained for
reference.
NOTE: Do not use the well factor file containing dynamic well factors (identified by the name
Dynamic_.....), since this file contains only the well factors for the wells that were used in the
experiment that generated this file. If the well list does not match, the operation will fail. Hence,
it is a good practice to only use the Persistent Well Factor files when applying alternate well
factor.