Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 66)

Section 6 Data Analysis Module
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Columns can be sorted by clicking on the column headings and reordered by clicking and
dragging to move columns. Data can be exported by right-clicking on the spreadsheet and
selecting Export to Excel. Data can be printed by right-clicking on the spreadsheet and selecting
Print. None of the data in this spreadsheet may be edited.
6.4.3 Amplification Data (RFU) Spreadsheet
You can view the individual RFU readings for each amplification trace at every cycle in the
Amplification Data (RFU) spreadsheet. Access the Standard Curve/C
T
Results spreadsheet by
clicking Results on the PCR Quant screen and selecting the Amplification Data (RFU) tab at the
bottom of the spreadsheet view (see Figure 6.11).
The spreadsheet can display data for only one fluorophore. The well number appears at the top
of the spreadsheet, and the cycle number appears down the side of the spreadsheet. In Single
Point mode, each cycle is represented by one point that is the mean of all the data points
analyzed during that cycle. In All Candidates mode, each individual data point displays.
6.5 Amplification Plot Context Menu
You can access PCR amplification plot data analysis and display parameters by right-clicking on
the PCR amplification plot. After you right-click on the plot, the amplification plot menu displays
(Figure 6.12).
Fig. 6.12. The Amplification Plot Menu.
6.5.1 Data Analysis Options
There are three additional data analysis options in the amplification plot menu:
•
Set Data Analysis Window
•
Digital Filter
•
BaseLine Threshold
Set Data Analysis Window
After you click Set Data Analysis Window in the Amplification plot menu, the Set Data Analysis
Window appears as shown in Figure 6.13. The number of data points collected per cycle depends
on the exposure and the dwell time of the cycle. You can change the percentage of data points
used in determining the RFU value, and you can choose to select this percentage from either the
beginning or the end of the cycle or from a window in the middle of the cycle.