Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 60)

Section 6 Data Analysis Module
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6.2.4 Selecting and Viewing Traces
•
Identifying a specific amplification trace: Identify a specific trace by moving the
mouse pointer along the trace until the hand icon appears. The dialog box identifies the
trace, by both well name and fluorophore, in the top left corner of the amplification chart
•
Selecting a specific amplification trace: Select a specific trace by moving the mouse
pointer on the trace until the hand icon appears, and then double-click. The dialog box
displays the selected trace. If you click the fluorophore selector buttons from the
amplification chart, traces from other fluorophores in the selected well also appear
• Viewing all traces: To restore a view of all traces right-click on the amplification chart
and select Show All Traces
•
Zoom: To zoom in on a section of the plot, click and drag with the mouse on the desired
region. To zoom out, select the plot and then type R, or right-click on the plot and then
click Restore Graph in the menu
Analyze Wells
Click Analyze Wells on the PCR Quant screen to select the wells that you wish to include in data
analysis. The Select Wells to Analyze floating window appears as shown in Figure 6.5.
Fig. 6.5. The Select Wells to Analyze Window.
You can include or exclude wells for analysis in this floating window. The original data are always
preserved, and excluded wells can always be added back to the data analysis in this window.