Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 127)

Section 7 Calibrating the Instrument
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7.4.5 Editing and Creating Pure Dye Plate Setups
Selecting a Pure Dye Plate Setup
Select a Pure Dye Plate Setup from the file selector window within the Pure Dye Calibration tab.
When loaded, the fluorophores on the plate will display within the plate display in the lower right-
hand corner of the Pure Dye Calibration tab.
Check the Seal Type and Vessel Type fields. If these fields match the intended procedure and the
correct fluorophores are displayed, proceed with pure dye calibration. If any of these particulars
vary from the intended experiment parameters, the Pure Dye Plate Setup must be edited or a new
Pure Dye Plate Setup created. An example of a Pure Dye Calibration Plate Setup window is shown
in Figure 7.6.
Fig. 7.6. Pure Dye Calibration Plate Setup Window.
Editing or Creating a Pure Dye Plate Setup
To edit a Pure Dye Plate Setup, select the Pure Dye Plate Setup that most closely resembles the
experimental parameters desired, and click Edit. The Pure Dye Plate Setup editor will load as
shown in Figure 7.7.
Fig. 7.7. Creating a Pure Dye Calibration Plate Setup.