Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 150)

Appendices
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Cause:
This is not an error message but a warning reminder that the iQ5 software is running in
emulation mode. In emulation mode the camera is not detecting and collecting data from the
plate and is generating emulated run data for demonstration purposes only.
Solution:
If you wish the camera to detect and collect data from your experimental plate, exit the software.
From the Start Menu, select programs > Bio-Rad > iQ5 >iQ5 to restart the software in standard
data collection mode.