Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 80)

Section 6 Data Analysis Module
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In Manual Call mode, the plot displays the RFU or threshold cycle data only. The threshold bars
do not appear, and modifications to calls are made manually by using the drop-down menu in the
Call column of the data spreadsheet. Alternatively, modifications to calls are made by choosing
the call type from the radio buttons in the Allele Call box and then clicking and dragging to select
desired samples in the graph.
You may zoom in on the allelic discrimination plot by first selecting Zoom Enabled from the plot
menu, then left-clicking and dragging over the area you wish to enlarge. Zoom out by choosing
Restore Graph from the context menu.
6.7.2 Allelic Discrimination Plot Menu
Open the allelic discrimination plot menu by right-clicking on the allelic discrimination plot (Figure
6.29).
Fig. 6.29. The Allelic Discrimination Plot Menu.
• Adjust Graph: This feature allows you to change the way that the plot is presented.
The maximum and minimum values for either axis may be entered directly into the text
boxes. Alternatively the up and down arrows can be used to set the maximum and
minimum values
• Restore Graph: Use this to redraw the graph after zooming
• Zoom Enable: Use this to enlarge the desired plot area
• Show Labels: Labels each sample on the Allelic Discrimination plot with its well name
• Copy Graph: This will copy the displayed Allelic Discrimination plot to the clipboard for
import into other programs
• Print Graph: This prints only the graph
• Print Data: This prints the Allelic Discrimination spreadsheet data
6.7.3 Allelic Data Spreadsheet
A six-column spreadsheet is displayed in the window (Figure 6.30). The first column is the well
number, and the second and third are the identifier entered at plate setup for the two
fluorophores. The fourth and fifth column present the RFU or threshold cycle data for both
fluorophores, and the last column is the genotype call made by either the software or the user.