Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 54)

Section 5 Run-Time Central Module
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3. If a multiplex assay is being performed, repeat steps 1 & 2 for all fluors present in Fluor
Selection list.
4. When finished, return to the Initiate Run screen.
5.6 Monitor Run Window
Open the Monitor Run window by clicking the Monitor Run tab. The window appears as shown in
Figure 5.5. Traces are displayed in real-time and run progress is monitored in the top left hand
section of the window.
Fig. 5.5. The Monitor Run Window.
5.7 Run-Time Protocol Editing
During a run, you can access real-time protocol editing options by selecting Run-time Protocol
Editing as shown below.
Selecting this checkbox activates the Next Cycle and Add 10 Repeats buttons as shown below.
• Next Cycle: Click Next Cycle to complete the current repeat of the present cycle
before skipping to the next cycle. For example, you could use this feature when your
samples have clearly crossed threshold and you want to skip to the melt cycle of your
protocol