Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 41)

Section 4 Workshop Module
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Fig. 4.13. The Protocol Selected Protocol Pane.
4.3.2 Editing or Creating a Protocol
To edit a selected protocol, double-click the protocol file or click Edit in the Selected Protocol
window. To create a protocol from a protocol template, click Create in the Selected Protocol
window. Clicking Edit or Create in the Viewing Protocol window will transfer the software to the
Protocol Editor.
You can edit the protocol in the spreadsheet at the bottom of the Editing Protocol window:
• Editing Dwell Time and Setpoint Temperature: Click in the spreadsheet in the
Dwell Time or Setpoint cell, then enter the dwell time or setpoint temperature
• Data Acquisition: Click in the spreadsheet in the Data Acquisition column, and select
real-time data collection for any step. Choose Melt Curve if data from a melt curve is
required
NOTE: Ensure that every thermal protocol has at least one data acquisition step.
• Inserting Cycles and Steps: To insert a cycle, click in the Insert column on the Cycle
row. Cycles are indicated with a blue background. To insert a step, click in the Insert
column on a Step row. Steps are indicated with a white background.
• Deleting Cycles and Steps: To delete a cycle, click in the Delete column on the Cycle
row. Cycles are indicated with a blue background. To delete a step, click in the Delete
column on a Step row. Steps are indicated with a white background.
•
Saving the Protocol: To save a protocol, click Save & Exit Protocol Editing. Enter
the name of the protocol in the Save As dialog box and click Save again