Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 116)

Section 6 Data Analysis Module
- 107 -
9. Select the type of replicate loading desired.
10. Click or drag across the plate to define wells with the selected fluorophore and sample
type.
11. Continue defining the remaining wells that will contain the first fluorophore by changing
to any other sample type icons required.
To calculate standard concentrations automatically, click Dilution Series and enter the
upper or lower concentrations and units of the standards, set the dilution factor, and
click Apply Dilution Series.
12. Repeat steps 7-11 for any additional dye layers/fluorophores as required. Remember that
if the Whole Plate Loading box is checked, changes made in standard concentrations will
be applied to all dye layers for that well and extended to all replicates in the group.
NOTE: To delete a previously defined well, click the Delete All icon, and then click the
well. To delete the selected fluorophore from a previously defined well, click the Delete
Fluorophore icon, and then click the well.
NOTE: Use the Next # checkbox to enter a particular number to assign to the next
standard or sample you define.
13. Click Apply Plate Changes to make the changes. To see the effect on analysis, go to one
of the other Data Analysis windows.
For more information about Edit Plate functions, refer to section 4.2.
6.12 The Reports Tool
The Reports tool of the iQ5 software is accessible from the menu bar of the iQ5 software.
Clicking on the heading for Reports will open the Report Viewer (Figure 6.64).
The Reports Viewer pulls data, charts, and graphs directly from the displayed settings of the Data
Analysis module currently in use. For this reason, it is best to complete all desired analysis and
formatting of the displayed data prior to using the Reports tool.
Fig. 6.64. The Report Viewer Window for Creating New Reports.