Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 88)

Section 6 Data Analysis Module
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Gene expression analysis is the most common real-time PCR application. Therefore, this manual
uses terminology that is specific to this kind of study. You can safely substitute “gene” with the
word “sequence” or “target” when you read about how to use the gene expression module.
Without stringently quantified controls, you cannot use this part of the iQ5 software to evaluate
target concentrations of particular sequences relative to each other. The iQ5 software can only
evaluate relative differences of a sequence between a group of samples.
The gene expression analysis screen has flexible tools for the determination of the fold induction
of one gene relative to another gene, or relative to itself under different circumstances; that is,
temporally, geographically, or developmentally different points. Additionally, within the gene
expression analysis screen, it is possible to create a Gene Study from multiple plates of data
collected at different times.
The gene expression analysis screen (Figure 6.38) consists of four main areas:
• The graphical data view section: Displays graphed data and graph settings
• The Settings and Data Table options: Located to the right of the graph section. Used
respectively to specify analysis settings and access the data spreadsheet
• The gene expression plate interface section: Located at the bottom of the screen. A
spreadsheet view of the plate setup used to modify and edit gene and condition names
• Analysis method radio buttons: Located above the gene expression plate interface
section. Used to select between normalized and relative quantity gene expression
analysis methods
Fig. 6.38. The Gene Expression Analysis Tab Window.