Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 148)

Appendices
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Cause:
Well factor data not found. This error occurs when a run start is attempted using a vessel and
seal combination the instrument has not been calibrated for.
Solution:
Well Factors vessel and seal type for your plate must match collected Persistent Well Factor
calibration files. The instrument should ideally be re-calibrated for the desired vessel and seal
type combination. If a run must be started immediately, select a vessel and seal type for which
the instrument has already been calibrated. After the run has completed recalibrate the
instrument for the desired vessel and seal combination and back-apply this new Well Factor
calibration to your data file. Refer to section 4.5.5 for further details.
Cause:
Pure dye calibration data not found. This error occurs when a run start is attempted using a
fluorophore with a vessel and seal combination the instrument has not been calibrated for.
Solution:
The instrument should ideally be re-calibrated for the fluorophore with desired vessel and seal
type combination. This is important for correct dye de-convolution in multiplex experiments.
NOTE: If a run must be started immediately, select a vessel and seal type for that fluorophore
for which the instrument has already been calibrated. After the run has completed recalibrate the
instrument for the desired vessel and seal combination and back-apply this alternate pure dye
calibration (.RME file) to your data file. Refer to section 4.5.4 for further details.