3 hydrating and conditioning the epm – Oritron Dionex Ion Chromatography System ICS-2100 User Manual

Page 185

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5 • Service

Doc. 065291-01 3/09

173

5.21.3 Hydrating and Conditioning the EPM

Follow the steps below to hydrate the EPM for 5 minutes and then
condition the EPM for 30 minutes.

1. Fill the eluent reservoir with ASTM filtered, Type I (18-megohm)

deionized water.

2. Verify that the suppressor and the auxiliary electrolytic device (if

installed) are off.

3. Verify that the EluGen cartridge is off.

4. Verify that the

Automatically turn on with pump

options are not

selected on the ICS-2100 touch screen

SUPPRESSOR

page (see

Section B.9

) and

ELUENT GENERATOR

page (see

Section B.8

).

Disabling these options lets you turn on the pump, without
automatically turning on the suppressor and EluGen cartridge.

5. From the ICS-2100 Control panel in Chromeleon or the ICS-2100

touch screen

HOME

page, set the pump flow rate to 1.00 mL/min.

6. Turn on the pump.

7. Run the pump for 5 minutes to hydrate the EPM.

8. Select the following settings from the Chromeleon Control panel or

the touch screen

ELUENT GENERATOR

page:

Set the

EGC-1

concentration to 9 mM.

Set the

EGC-2

concentration to 1 mM.

9. Turn on the EGC-1 and EGC-2 currents.

10. Run at these settings for 30 minutes.

11. Turn off the EGC-1 and EGC-2 currents.

12. Turn off the pump.

13. Remove the backpressure coil and couplers from the

TO INJ VALVE IN

- P/ELUENT OUT

and

TO SRS/AES REGEN OUT

lines.

To avoid damaging the suppressor and auxiliary electrolytic device (if
installed), always turn them off before conditioning the EPM and
EluGen cartridge. The pump flow is on during conditioning; however,
no flow reaches the suppressor or auxiliary electrolytic device.

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