Bio-Rad BioLogic Signal Import Module (SIM) User Manual
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1.2
FEATURES
BioLogic DuoFlow systems provide the following features:
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Setup Flexibility. A space saving modular design that is stackable and easily configured to meet
your exact needs and fit into your desired bench space. Trays and vertical bars are moveable and
removable. Horizontal bars can be placed in the optimal position for valves, columns, detectors, etc.
The system fits easily into a cold box.
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Modular components provide an easy upgrade path to fit all applications and financial
requirements. For example, the BioLogic DuoFlow™ Basic system may be purchased and upgraded
to a BioLogic DuoFlow QuadTec™, BioLogic DuoFlow Maximizer or BioLogic DuoFlow Pathfinder
system as needed. (See Section 1.4 for a description of the DuoFlow systems and upgrades.)
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Intuitive, user-friendly software programming. Users become an expert in only a few runs.
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Four easy steps to set up new devices/instruments, create a new method, and start to run
samples:
Step 1. Browser, to enter new user name, project name and method name.
Step 2. Setup, to specify devices required for the method.
Step 3. Protocol, to enter sequential method steps.
Step 4. Run, to inject sample and view the real time chromatogram.
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Two easy steps, if you want to run a sample using a current Setup and Protocol:
Step 1. Browser, to select a user and along with a method or Method Template.
Step 2. Run, to inject sample and view real time chromatogram.
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On screen Help. Includes detailed information and a troubleshooting guide.
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USB Bitbus Communicator. The USB Bitbus communicator allows the BioLogic DuoFlow system
to be controlled from any computer running Windows 2000 and BioLogic software 4.0 or above, over
a USB port.
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Buffer Blending. Buffer Blending is a feature of the BioLogic DuoFlow Maximizer™ and Pathfinder
systems that dynamically "Blends" the conjugate acid and base of a buffer with water and salt to
produce a solution with a specific salt concentration and pH.
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Buffer Editor. The Buffer Editor is a feature used to create buffer systems for use in Buffer Blending
experiments. Both single and multiple component buffers can be created.
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Scouting. This feature facilitates the optimization of a chromatography protocol for a specific target
molecule. Scouting systematically increments a user selected variable and then performs a
chromatography run at each increment. Variables that can be "Scouted" include: pH, %B, step
duration, column, buffer, flow rate, sample and sample volume.
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Method Templates. Includes ready-to-run chromatography protocols for a variety of experiment
types including: affinity, chromatofocusing, hydroxyapatite, hydrophobic interaction, ion exchange
and gel filtration chromatography.
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pH Monitor. The BioLogic pH monitor allows direct pH monitoring during a run. It is included as part
of the DuoFlow Maximizer and Pathfinder systems and is available as an option for all other system
configurations. The pH monitor consists of a Calomel Tris compatible electrode in a PEEK
biocompatible flow cell.
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Flow Rate Flexibility. The DuoFlow Workstation has two pump head options (F10 and F40) that
permit a wide range of flow rates (0.01 ml/min up to 80 ml/min).
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Detection Flexibility
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UV detector with fixed 254 nm and 280 nm filters, long life mercury lamp, and additional drop-in
expansion filters available.
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UV detector can be expanded to 214 nm filter with zinc lamp.
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QuadTec UV/Vis detector analyzes samples simultaneously at 4 different wavelengths from 190-
370 nm with a deuterium lamp or 370-790 with a halogen lamp.
INTRODUCTION
SYSTEM OVERVIEW
1-2