Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 134)

7.2.1 Device Selection
The devices that can be defined in the device setup are described below. The actual devices available on
your DuoFlow system may vary. All DuoFlow systems are delivered with a detector, conductivity monitor and
an AVR7-3 valve. All other devices are optional (See Appendix D for information about ordering additional
devices).
• Auxiliary Pump: Used to define the type of auxiliary pump connected to the DuoFlow system:
Econo Gradient Pump (EGP), Model EP-1 Econo or generic (non-Bio-Rad) pump. These pumps
may be defined as a sample load pump or as a user-defined pump (EGP only). See Chapters 2 and
8 for ways to use an auxiliary load pump. Generic pumps should be defined as a Model EP-1 pump.
• Fraction Collector: Used to define the type of fraction collector connected to your system: BioFrac,
Model 2128, Model 2110 or generic (non-Bio-Rad). The fraction collection options available depend
on the type of fraction collector connected to the system. All of the fraction collectors can be
synchronized with a detector signal by checking "Synchronize Fraction Collection with the Detector"
and entering a delay volume. The delay volume is defined as the system fluid volume (including
tubing and inline devices) between the significant detector and the fraction collector drop head. See
the BioLogic software online Help for help determining the delay volume.
The BioFrac and Model 2128 options are used to control these fraction collectors over the system
bus and to set the rack type, collection pattern (BioFrac only) and whether or not the maximum
number of racks may be exceeded. If you choose to exceed the maximum number of available racks,
the run will pause when the rack(s) are full and prompt you to add new racks. The BioFrac or Model
2128 fraction collector must be selected in the BioLogic Configuration Utility (see Chapter 3.11.3)
before starting the BioLogic software.
The Model 2110 and generic options allow you to control these fraction collectors over the Aux port
on the rear of the BioLogic Workstation or Maximizer. If a Maximizer is connected to your system
then the fraction collector must be connected to it and not the Workstation. Note, a SVT3-2 valve
must be connected to your system and defined as a diverter valve in the device setup if threshold or
windows collection is desired.
• Buffer Blender: Used to place the DuoFlow system in Buffer Blending mode, select a pre-defined
Bio-Rad or user-generated buffer system, set pH corrections and view solution preparation
instructions, pH range and buffer temperature coefficients. The Buffer Editor feature is used to
generate user-defined buffers for Buffer Blending (see Chapter 7.2.3).
• Detectors: Used to define the detectors types used by a method. Up to six detector traces may be
defined including: UV Detector, QuadTec (up to four traces), Conductivity, SIM pH and SIM signal
(up to two external detector traces such as refractive index or fluorescence detectors). The external
detectors are connected through a SIM-HR and/or a Maximizer SIM (see Chapter 2.9.6 and
Table 2-7, respectively).
• Valves (SVT3-2, SV5-4, AVR7-3 and AVR9-8): Used to define valve functionality for 3 solenoid and
3 motorized valves (6 solenoid and 6 motorized valves if a Maximizer is installed with your system).
The valve setup dialogs are used to define the valve name and function, specify which port the valve
is connected to and to assign a name/function to each valve position. See Table 7-1 for valve
function options.
MODES OF OPERATION SYSTEM OPERATION
7-5