Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 182)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
9-1
9.0 COLUMN AND BUFFER FLOW SWITCHING APPLICATIONS
9.1 COLUMN SWITCHING
A Column Switching valve is used to connect multiple columns to the BioLogic DuoFlow system. Inclusion of
a column-switching valve in the setup can be an advantage if you routinely use a variety of columns on your
system. One application of this feature includes the running of multiple samples (see Section 8.1 and 8.2)
where each sample requires its own column. Another application is the running of two and three- dimensional
chromatography experiments where the sample eluted from one column is subsequently injected onto a
second or third column. There are two types of column switching valves that can be used with the DuoFlow
system: AVR7-3 two-column switching valve and an AVR9-8 eight-column switching valve (requires 2 AVR9-8
valves).
9.1.1 AVR7-3 Two-Column Switching
Figure 9-1. Column Switching Using Two AVR7-3 Valves
For this application, an AVR7-3 column-switching valve is required in addition to the AVR7-3 sample inject
valve.
System Setup
1. Plumb an AVR7-3 column-switching valve as shown in Figure 9-1. Column 1 should have its inlet
connected to port 4 and outlet to port 3. Column 2 should have its inlet connected to port 6 and
outlet to port 1.
2. Plumb port 5 of the column-switching valve to port 4 of the upstream sample inject valve.
3. Plumb port 2 of the column-switching valve to your detector.
4. In the device setup, configure the AVR7-3 valve as a user-define valve and name the valve "Column
Switcher". Name valve positions 1, 2 and 3 as Column 1, Column 2 and Purge, respectively.
WORKSTATION
A B
1
AVR7-3
AS INJECT VALVE
AVR7-3
AS COLUMN SWITCHING VALVE
MIXER
SAMPLE
LOOP
UV DETECTOR AND
FRACTION COLLECTOR
SAMPLE
INJECT
6
7
1
2 3
4
5
LOAD
COLUMN
#1
COLUMN
#2
6
7
2 3
4
5
LOAD