Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 69)

• Macro-Prep ® CHT Support: Macro-Prep ® ceramic hydroxyapatite (CHT) overcomes the physical
and chemical instability of crystalline hydroxyapatite and is available in two types. Type I has a high
protein binding capacity and better binding capacity for acidic proteins. Type II is better suited for
proteins that elute early and for nucleic acids. Refer to bulletins 1842 (C-100), 1927, 1971, and
2156.
2.10.6 Size Exclusion Chromatography (SEC)
The SEC supports are available in the following formats:
• HPLC SEC Columns: Bio-Sil and Bio-Select 5 µm silica-based columns separate compounds by
the mechanism of size exclusion. The technique is based on diffusion in and around highly porous
spherical silica beads. The columns are recommended for the separation of peptides, proteins, and
nucleic acids, for desalting or buffer exchange, and for molecular weight or molecular constant
determination. Columns are available in both stainless steel (Bio-Sil columns) and biocompatible
PEEK plastic hardware (Bio-Select columns). Each column is shipped with a free vial of Bio-Rad’s
protein standards. Refer to bulletins 1737 and 1946.
• Econo-Pac P6 Low Pressure Chromatography Cartridges: This 5 ml cartridge is used for
desalting and buffer exchange. Refer to bulletin 1946.
2.10.7 High Pressure Reversed Phase Columns
The following type of column is available:
• Hi-Pore reversed phase columns: These columns are commonly used for the purification and
analysis of small proteins (<50 kd), peptides, oligonucleotides, and amino acids. Refer to bulletin
1946.
2.10.8 Hydrophobic Interaction Chromatography (HIC)
The HIC chemistry is available in the following formats:
• Econo-Pac t-Butyl HIC Low Pressure Chromatography Cartridges: These cartridges are
available in a 5 ml format to accommodate most sample loads. They are recommended for method
scouting and for first step purification of crude samples. They are based on 50 µm Macro-Prep
®
supports. Refer to bulletins 1946 and 2079.
• Econo-Pac HIC Low Pressure Chromatography Cartridges: HIC is offered in both methyl and
t-butyl chemistries. These cartridges are available in 1 ml and 5 ml formats to accommodate most
sample loads. They are recommended for method scouting and for first step purification of crude
samples. They are based on 50 µm Macro-Prep supports. Refer to bulletin 1946.
• Macro-Prep ® HIC Support: HIC is offered in both methyl and t-butyl chemistries. The Macro-Prep ®
methyl HIC support is ideal for purification of proteins with strongly hydrophobic regions. The
Macro-Prep
® t-butyl HIC support is ideal for purification of proteins with few or weakly hydrophobic
regions. Refer to bulletin 1841.
DESCRIPTION OF SYSTEM COMPONENTS SYSTEM OVERVIEW
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