Bio-Rad BioLogic Signal Import Module (SIM) User Manual
Page 181
SAMPLE LOADING
ADVANCED SYSTEM APPLICATIONS
8-8
b. Select Fill Before Inject. This instructs the auxiliary pump to load the sample into the
DynaLoop. Note that the auxiliary pump flow rate is not under DuoFlow control. The flow rate is
used by the system when validating the protocol before the run. Because the DuoFlow system
only starts/stops the auxiliary pump, the correct flow rate must be set at the auxiliary pump. The
flow rate of the Econo pump is set from the pump and is recorded in the yellow data entry boxes
of the Fill Sample section of the Load/Inject Sample dialog box. Note that the rinse function is
not available when the DynaLoop is being used.
c. In the Fill Sample Loop area of the window, select the sample to be loaded and enter its volume
and flow rate of the auxiliary pump.
d. In the Inject Sample area of the window, select the Injection Buffers, the buffer composition, the
flow rate of the Workstation pumps, and the sample volume to be injected onto the column.
The DuoFlow system will now automatically control the loading and injection of the sample. Note
that the rinse function is not available when the DynaLoop is being used.
5.
Continue writing your desired separation protocol.
During the run, the valve functions as follows:
•
Load. While in this position the valve connects ports 5 and 4 for equilibration of the column and for
sample elution. In this position, sample loop is loaded to the desired volume via port 2 and buffer is
expelled from the dynamic loop through port 1.
•
Inject. While in this position the valve connects ports 5 and 6 and ports 3 and 4 for applying the
sample onto the column. The flow from the Workstation pump forces the sliding piston to expel the
sample onto the column.
•
Purge. While in this position the valve connects ports 5 and 7 and allows purging or buffer changes
of the Workstation pump without the need to remove the column from the system.
Figure 8-6. Valve Positions During a Run using the DynaLoop
DYNAMIC
LOOP
WORKSTATION
PUMP
DYNAMIC
LOOP LOADING
WASTE
WASTE
6
7
1
2
3
4
5
LOAD
WASTE
WASTE
6
7
1
2
3
4
5
INJECT
WASTE
WASTE
6
7
1
2
3
4
5
PURGE
AUXILIARY
LOAD PUMP
OR
DYNAMIC
LOOP LOADING
AUXILIARY
LOAD PUMP
OR
DYNAMIC
LOOP LOADING
AUXILIARY
LOAD PUMP
OR
COLUMN
DYNAMIC
LOOP
WORKSTATION
PUMP
COLUMN
DYNAMIC
LOOP
WORKSTATION
PUMP
COLUMN