Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 170)

7.5.4 Entering Activity Data
The Activity Trace Editor permits data collected by a separate offline method to be included with data
collected by the DuoFlow method. For example, if fractions collected by the DuoFlow are also analyzed by
an ELISA method or for radioactivity, CPM, or DPM, the data collected by these assays can be entered into
the Activity Trace Editor and a trace will appear on screen reflecting the activity for each fraction.
1. To enter activity data: Select the run for which you wish to enter the post run activity data from the
Browser screen.
2. Press the Activity toolbar button. A box will appear listing each fraction collected in the opened run.
(See Figure 7-13.)
3. Enter activity values for each fraction and enter the desired units.
Check the Show Activity Trace box to display the data in the Post Run screen.
4. The activity values entered will appear in the chromatogram information screen and as a trace in the
chromatogram. (See Figure 7-14.)
Figure 7-13. Activity Trace Editor
Current Tagging Trace:
QuadTec-3 (214 nm)
Run Time:
Gradient:
QuadTec-1 (280 nm):
QuadTec-2 (260 nm):
QuadTec-3 (214 nm):
QuadTec-4 (405 nm):
Conductivity:
GP pressure:
No Trace:
00:03:38.4
21% Buffer B
0.0345 AU
0.0228 AU
0.4762 AU
0.0021 AU
8.47 mS/cm
375.53 psi
0.080
50
100% Buffer B
QuadTec-3 (214 nm)
0.100
0.090
00:00:00 00:02:00
2
3
4
5
00:04:00 00:06:00
Hr:Min:Sec AU psi
GP pressure
0.070
0.060
0.050
0.040
0.035
0.020
0.010
0.000
-0.010
100.0
0.0
90.0
80.0
70.0
60.0
50.0
40.0
30.0
20.0
10.0
-10.0
Fractions
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
Split
1.00ml/min 0 %B
Gradient Pump: F40 UV Conductivity
438 psi 0.03775 AU 359 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.953 Volt 0.00 pH
Activity Trace Editor
Activity
Fraction
#
Fraction
start time
A, 1F
A, 2F
A, 3F
A, 4F
A, 5F
Show activity trace Reset Activity Values
00:00:00
00:00:30
00:01:00
00:01:30
00:02:00
Fraction
end time
Activity
value
Activity units
00:00:30
00:01:00
00:01:30
00:02:00
00:02:30
0.00 dPm
500000.00
0.00
700000.00
0.00 Cancel
OK
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
File Edit View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup Protocol
wash
load
1
2
Run Notes Report PostRun Log
Window Help
Settings
Values at Cursor
Tags
AU
SA
FullView Print Del Tag Activity
Bio-Rad
Web
MODES OF OPERATION SYSTEM OPERATION
7-41