Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 176)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
8-3
pump inlet valve may be used to load 1 samples or 3 samples, respectively.
System Setup
1. Connect an auxiliary pump to the system as described in Section 3.9.1 (EP-1 or non-Bio-Rad pump)
or 3.9.2 (Econo Gradient Pump).
2. Plumb the Aux Pump to port 3 of the AVR7-3 Sample Inject valve as shown in Figure 8-2. If an
auxiliary pump inlet valve is connected to the system it should be plumbed between the load pump
and the AVR7-3 Sample Inject valve.
3. In the device setup screen, add an Aux Pump to the setup and define it as a load pump. Define non-
Bio-Rad pumps as an EP-1 load pump.
4. (Optional) If an Aux Pump Inlet valve is used, add the valve to the device setup, define the valve as
an Aux Pump Inlet valve and name each position. One position (usually position 1) should be
defined as a "Rinse" solution. The rinse solution is used to clean the sample loop between
injections. Note that some sample will be lost in the process of pulling sample from a remote beaker.
Be sure to factor in the solution volume required to fill all the buffer lines leading up to the top of the
column when programming the injection step.
5. Remove the 40-psi backpressure regulator from the post-column position and place it between the
Workstation pump and the mixer. This allows low-pressure peristaltic pumps to push sample through
the column while at the same time will help the check valve to seat properly and ensure pump flow
performance. In addition, low-pressure columns may burst if the backpressure regulator is placed
after the column.
6. Plumb the Aux Pump to port 3 of the AVR7-3 Sample Inject valve as shown in Figure 8-2.
7. (Optional) If an Aux Pump Inlet valve is used, connect it to the Aux pump inlet.
Figure 8-2. Plumbing an AVR7-3 Inject Valve with an Auxiliary Load Pump
Writing the Aux Pump Direct Inject Protocol
Several methods that include the Aux Pump Direct Inject feature have been included in the BioLogic
DuoFlow Method Templates (see Section 6.2 and the online help for more information). This feature may be
added to any protocol.
1. In the protocol load/inject step select Direct Inject as the injection type.
2. Select the Aux Load Pump option and set the sample volume, flow rate and flow direction. Note that if
an EP-1 or non-Bio-Rad pump is used, the flow rate must be set both in this dialog and at the pump.
3. If an Aux Pump Inlet valve is connected, select the sample to load.
4. If the tubing is to be rinsed between sample injections add a second Load/Inject step, select the
rinse buffer and set the rinse volume, flow direction and flow rate.
LOW
PRESSURE
COLUMN
AUXILIARY
LOAD PUMP
PURGE
AUXILIARY
LOAD PUMP
WORKSTATION
PUMP
WASTE
WASTE
6
7
1
2
3
4
5
AVR7-3