Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 100)

4.3 PRIMING THE SYSTEM
Before a method can be run, the system must filled with buffer and all air bubbles purged from the system.
The following procedure should be used to prime the system for the first time and when changing buffers.
1. Priming the Workstation pump.
a. Immerse the Workstation pump A and B or Maximizer A1, A2, B1 and B2 inlet lines into a
container of HPLC grade (filtered, degassed) or other high quality water.
b. Place the 10 ml luer syringe (supplied with the fittings kit) in the priming port of pumphead A. If a
Maximizer is connected, select Inlet A1.
c. Turn the priming port counter-clockwise to open the port and gently draw water into the syringe
from the pumphead.
d. Repeat this operation until no air bubbles are visible in the inlet tubing.
e. Disconnect the pumphead outlet tube and hold a beaker up to the port. With the syringe full of
water inject water into the priming port using several short pulses to dislodge any trapped air
bubbles. Once all the bubbles have been dislodge, close priming port and reconnect the
pumphead outlet tube.
f. Repeat this priming procedure for the pump B inlet or inlets A2, B1 and B2, if a Maximizer is
connected.
2. Flush the System Through to the Fraction Collector.
a. Take the column out of line if it has been connected.
b. From the Manual screen, place the AVR7-3 injection valve into the Purge position. The AVR7-3
valve control panel is labeled with the valve type and the Workstation port where the valve is
connected. For example, if the valve is connected at Workstation port 4, the panel will be named
"AVR7-3 at port 4." The AVR7-3 valve the panel shows three valve positions Load (L), Inject (I)
and Purge (P).
c. Press the Purge buttons A and B on the front of the Workstation. The Workstation pump will run
and the indicator lights will flash green. The default purge flow rate is 10 ml/min for F10 pumps
and 40 ml/min for F40 pumps. The default purge flow rate can be changed from the
Options/Manual Setup menu option on the Manual screen.
d. Run both pumps for about 2 minutes. If a Maximizer is connected to the system, place the valve
inlets at position A2 and B2 for about a minute and then at A1 and B1 for an additional minute.
Press the Purge buttons again to stop the pump.
e. From the Manual screen place the AVR7-3 injection valve into the Inject (I) position.
f.
Set the pump flow rate to 1.0 ml/min and start the pump. Water will now flow through the sample
loop, the UV Conductivity flow cells, and ultimately to the fraction collector.
g. After the system is filled, change the AVR7-3 injection valve to Load and monitor the pressure
displayed on the status bar for a few minutes. If the pressure variation exceeds ± 10 % repeat
step 1e until the pressure is stable. It is best to monitor the pressure with some backpressure on
the pumps (such as the 40 psi backpressure device). See Section 11.3 for additional
information.
SYSTEM PLUMBING SYSTEM INSTALLATION AND SETUP
4-8