Bio-Rad BioLogic Signal Import Module (SIM) User Manual

Page 50

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2.7.2 Model 2110 Fraction Collector

The Model 2110 is programmed in the DuoFlow software and can function as a stand-alone fraction collector
with non Bio-Rad systems. It uses a stationary, drop-dispensing head and collects up to 80 fractions in a
motor-driven carousel. It also uses standard 13 x 100 test tubes. An optional adapter is available for use
with 1.5 ml microcentrifuge test tubes. The Model 2110 includes the following features:

In stand-alone mode the Model 2110 accepts small chromatography columns that can mount directly
to the drop-former to minimize dead volume.

It can collect from 1 drop (50

µl) to 9 ml fraction in the 13 x 100 test tubes or 1.5 ml micro test

tubes.

It is coldroom compatible.

Figure 2-20. Model 2110 Fraction Collector with Optional Dust Cover

The Model 2110 is connected to the Maximizer or Workstation with system cable 5, bare wires to DB-9 via
the AUX connector, black wire to pin 5 and white wire to pin 9. To prevent shorting, cut or tape wires not in
use. If a Maximizer is being used, the Model 2110 should be connected to it rather than to the Workstation.

The Model 2110 is plumbed to the DuoFlow system using 1/16” (1.6 mm) OD inlet tubing connecting directly
to the fraction collector’s drop former, without the need for additional fittings.

The SVT3-2 diverter valve is required by the Model 2110 for collection schemes other than “Collect All.”
When this valve is configured as the fraction collector diverter valve in the Setup screen, additional
collection parameters (Collect All, Threshold, Collection Windows, and Threshold + Collection Windows)
become available in the Protocol screen.

When controlled by the DuoFlow system, fraction collection is specified by volume. The fraction collection
scheme is programmed in the Protocol screen of the software where a Delay Volume feature is standard
(see Chapter 7 for discussion of Delay Volume.)

Note: Collection by drop count is not available with the DuoFlow system.

This instrument is described in detail in its separate documentation.

MODEL 2110 FRACTION COLLECTOR

DESCRIPTION OF SYSTEM COMPONENTS

SYSTEM OVERVIEW

2-37

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