Modes of operation system operation 7-9 – Bio-Rad BioLogic Signal Import Module (SIM) User Manual

Page 138

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Table 7-2

Buffer Editor

The Buffer Editor main screen is used to view information about currently defined buffer system and to
create and edit buffer systems.

Create New Buffer System: This button starts the Buffer System Wizard that is used to create new
buffer systems. Buffers and salts that are defined in the current buffer and salt lists can be used in a
buffer system.

Edit/View Buffer System: This button starts the Buffer System Wizard and is used to edit, view or
copy existing buffer systems. Buffers and salts that are in the current buffer and salts lists can be
used in a buffer system.

Edit Buffer List: This button starts a dialog used to create buffer salts that are used in buffer
systems.

Edit Salt List: This button starts a dialog used create salts that are used in buffer systems.

Buffer Editor Report: This button allows you to print out buffer system information.

Export Buffer System: This button allows you to export buffer system information to a text file.

Close: Exits the Buffer Editor.

Help: Opens the Buffer Editor online help.

Delete Buffer System: Deletes the currently selected buffer system. Bio-Rad buffer systems cannot
be deleted.

View Table: Used to display the pH as a function of % Inlet A2 and %B. Used to determine the pH
range for a buffer. The buffer pH range depends on the salt concentration and should be limited to
the table pH valves shown between 10% and 90% A2.

Buffer Editor

*Acetate (20 mM)

Buffer System:

Name:

2X Concentration:

Buffer(s)

Acetate

Buffer System:

Acetate

M

0.04

Name:

2X Concentration:

Salt

Sodium Chloride

M

2.00

M

M

3.60

5.60

25.0

(C)

Bio-Rad

Author:

pH Range:

to

at

Reference Temperature

Recipe Text

Acetate Acid (40 mM)
Prepare by dissovling:
80 ml of 1M Acetic Acid
in water and diluting to 2.0 L.

Sodium Acetate Trihydrate (40 mM)
Prepare by dissovling:
10.9 g of Sodium Acetate Trihydrate (MW 136.1 g/mol)
in water and diluting to 2.0 L.

Sodium Chloride (2.0 M)
Prepare by dissovling:
233.6 g of Sodium Chloride (MW 58.4 g/mol)
in water and diluting to 2.0 L.

Degassed Water
Degass water under vacuum for at least 15 minutes while stirring

Create New
Buffer System

Edit Current
Buffer System

Read Only

Buffer System
Report

Export Buffer
System

Delete Buffer
System

View Table

Edit Buffer

List

Edit Salt List

Close

Help

MODES OF OPERATION

SYSTEM OPERATION

7-9

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