Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 35)

Two flow cells are available with the detector. Both use 1/4-28 flat-bottom fittings.
• Analytical 5 mm flow cell. This flow cell is recommended for high resolution detection. This flow cell
has a 5 mm path length, a volume of 16
µl, and is rated to 750 psi at flow rates between 0.1 and 10
ml/min.
To reduce the risk of an entrapped air bubble causing an unstable baseline, the 5 mm flow cell
should be used with the backpressure regulator (see page 2-52). Connect the backpressure
regulator after the Conductivity monitor in the system plumbing.
• Preparative 2 mm flow cell. This flow cell is recommended for most applications which demand less
sensitivity, for flow rates greater than 10 ml/min, or when working with high protein concentrations. It
has a 2 mm path length, a volume of 30
µl, and is rated to 750 psi.
The UV detector receives power via the Workstation, to which it is connected by the UV lamp cable. The UV
detector communicates with the system via the UV optics cable, which plugs into the UV optics connector on
the back of the Workstation.
The UV detector sensitivity ranges from 0 to 2.0 AUFS (Absorbance Units Full Scale). The UV sensitivity
range for the chart recorder is set in the system software in either the Manual or Run screens. A Zero
Baseline button is available in the Manual and Run screens and a programmable Zero Baseline command is
available as part of a method protocol. Refer to the discussion of the chart recorder for setting the chart
recorder range, page 2-55.
To replace an expired lamp, refer to Chapter 11, Maintenance, or to the instruction sheet for the replacement
lamp.
Figure 2-6. UV Detector, with Zinc Lamp, 214 nm Filter, and Conductivity Flow Cell
To change the UV flow cell or UV filter:
If the system has been used, make sure that any hazardous material has been flushed from the system
and the pumps are not running. Drain fluid from the UV detector and disconnect its plumbing and
cables.
1. To remove the UV flow cell, loosen the flow cell thumbscrews and lift the flow cell out.
2. To insert a flow cell, place it into the UV detector and tighten the thumbscrews.
3. To change the UV filter, loosen the flow cell thumbscrews and lift the filter tray out.
4. Rotate the filter tray to use the correct filter, and then place it into the UV detector and tighten the
thumbscrews.
DESCRIPTION OF BIOLOGIC DUOFLOW SYSTEM SYSTEM OVERVIEW
2-22