Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 157)

Table 7-11 (continued)
Scouting
Creating a Scout Experiment
Setting up a Scout Method
1. Connect and plumb all the required hardware components to the DuoFlow system.
2. Define the required devices in the device setup.
3. Prepare sufficient buffers for the number of runs to be performed
4. Prime the system and equilibrate the column(s) that will be used
Adding a Scout Step
1. Write a new method, copy an existing method or use a Bio-Rad method template. This method will
be used for the first scout run. Make sure all parameters are set correctly and the necessary devices
have been defined in the device setup. Once a Scout step has been added to a protocol, it cannot
be modified unless the scout step is deleted.
2. Press the Scout button on the lower-left corner of the protocol screen to add a scout step to the
protocol using the Scout Wizard.
3. In the Scout Wizard Step 1 dialog, select the parameter type to be scouted. As a general rule, those
parameters that are expected to have the greatest impact on the chromatography results should be
scouted first.
4. In the Scout Wizard Step 2 dialog, select the protocol step or steps to be scouted. Steps that cannot
be scouted are grayed out, and steps selected for scouting are highlighted.
5. In the Scout Wizard Step 3 dialog, enter the number of runs, name the runs and for each run enter a
value for the scouted parameter. The run name can be auto-generated or entered manually.
Automatically generated run names use the Run 1 name as the base name. The value of the scout
variable can be auto-generated or entered manually. Auto-generated values use the Run 1 value as
the start value and increment the value by the Increment Value amount. The Increment Value can be
a positive or negative number and is always defined as 1 when scouting valve positions (e.g. Buffer
A, Buffer B, Sample Name and Column).
MODES OF OPERATION SYSTEM OPERATION
7-28