Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 67)

2.10 COLUMNS AND COLUMN FITTINGS
Bio-Rad offers a variety of column chemistries and formats. The following pages provide a summary of the
different column types and column fittings for use with BioLogic DuoFlow systems. Bio-Rad columns for the
DuoFlow use the following matrices:
• UNO TM columns use the new Continuous Bed matrix which contains an advanced polymer matrix
that is completely homogeneous. These columns are superior to beaded supports in resolution,
binding capacity, speed, and value.
• Bio-Scale chemistries are based on 10 µm Macro-Prep ® supports. They are offered in various sizes
and allow for scale-up separation and purification. These medium-pressure columns are ideally
suited for use on the DuoFlow system.
• The Econo-Pac cartridge chemistries are based on 50 µm Macro-Prep ® supports and are ideal for
first step purification.
2.10.1 Anion Exchange: Q Strong Anion Exchange
The Q strong anion exchanger chemistry is available in the following formats:
• UNO Q Biochromatography Columns: These columns are designed to handle separations at high
flow rates with low back pressure. Instead of a traditional bed of packed beads or particles, each
column contains an advanced polymer matrix, called the Continuous Bed matrix, which is nonporous
and homogeneous. The matrix is designed to maximize resolution, binding capacity, and speed.
Refer to bulletins 2116 and 1946.
• Bio-Scale Q Prepacked Medium Pressure Columns: These columns are designed for high
resolution separations of proteins, peptides, and polynucleotides in analytical to semipreparative
medium pressure applications. They are available in four column sizes. Methods developed on the
Bio-Scale columns can be transferred to production scale using the Macro-Prep
® supports. Refer to
bulletins 1880, 1946, and 2079.
• Econo-Pac High Q Low Pressure Chromatography Cartridges: These cartridges are available in
1 ml and 5 ml formats to accommodate most sample loads. They are recommended for method
scouting and for first step purification of crude samples. They are based on 50 µm Macro-Prep
®
supports. Refer to bulletin 1946.
• Macro-Prep ® High Q Support: This is a strong anion exchanger containing quaternary amine
functional groups with a 50 µm particle size. It is ideal for rapid purification of acidic and neutral
proteins and peptides. Refer to bulletins 1840 (A-100), 1917, and 1985.
2.10.2 Cation Exchange: S Strong Cation Exchange
The S strong cation exchanger chemistry is available in the following formats:
• UNO S Biochromatography Columns: These columns are designed to handle separations at high
flow rates with low back pressure. Instead of a traditional bed of packed beads or particles, each
column contains an advanced polymer matrix, called the Continuous Bed matrix, which is nonporous
and homogeneous. The matrix is designed to maximize resolution, binding capacity, and speed.
Refer to bulletins 2116 and 1946.
• Bio-Scale S Pre-packed Medium Pressure Columns: These columns are designed for high
resolution separations of proteins, peptides, and polynucleotides in analytical to semipreparative
medium pressure applications. They are available in four column sizes. Methods developed on the
Bio-Scale columns can be transferred to production scale using the Macro-Prep
® 50 µm supports.
Refer to bulletins 1881, 1946, and 2079.
DESCRIPTION OF SYSTEM COMPONENTS SYSTEM OVERVIEW
2-54