Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 184)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
9-3
System Setup
1. Plumb two AVR9-8 valves as shown in Figure 9-2. Each column inlet should be connected to the
column inlet valve and each column outlet to the column outlet valve. Unless eight columns will be
connected, a length of tubing should be plumbed between the two AVR9-8 valves at position 1 to be
used as a column bypass. This facilitates washing of the system between column switching events.
Column bypass tubing or ¼-28 plugs should be placed at each unused valve port.
2. Plumb the common port of the column-switching inlet valve to port 4 of the sample inject valve.
3. Plumb the common port of the column-switching outlet valve to your detector.
4. In the device setup, define an AVR9-8 valve as a Column-Switching valve. In the AVR9-8 valve
dialog identify the ports where the two AVR9-8 valves are connected to the Workstation or
Maximizer and name each valve positions, e.g. "Column Bypass", "Column 1, "Column 2" etc.
Writing the Column Switching Protocol
When using multiple columns, care should be taken to prevent cross-contamination. Purge steps should be
included before placing a column inline to ensure that the tubing is clean and filled with the appropriate
buffer. The following three steps should be included as part of a column-switching step.
1. Add a Column Switching step to your protocol and select the Column Bypass position.
2. Add an isocratic flow step to wash the tubing and mixer with 3-5 volumes of buffer.
3. Add a Column Switching step to your protocol and select the desired column.
Column-switching steps are usually placed at the beginning of a protocol or at the start of each dimension in
a multi-dimensional chromatography experiment.