Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 142)

Table 7-4. (continued)
Load/Inject Sample
The following selections are available in both dialogs.
• Load/Inject Sample Tab: Selects injection type. The AVR7-3 sample inject valve will move to the Inject
position at the start of the step and return to the Load position at the end of the step.
Static Loop: Standard fixed volume loop for sample loading. Used with the AVR7-3 valve, sample can
be loaded with a syringe through port 2, and the valve can rinse the sample. An auxiliary (AUX) pump
and valve (such as the SVT3-2, SV5-4, or AVR9-8), may be used to fill the loop on the AVR7-3 prior to
injection onto the column. Refer to Chapter 8 for discussion of how to select volume fill and rinse.
Dynamic Loop: Uses Bio-Rad’s DynaLoop or other sliding-piston sample loop. Both partial loop and full
loop injections can be programmed. The dynamic loop can be filled manually through Inject valve port 2.
An auxiliary (AUX) pump and valve (such as the SVT3-2, SV5-4, or AVR9-8), may be used to fill the loop
on the AVR7-3 prior to injection onto the column. The Rinse function is not available. See Chapter 8 for
DynaLoop loading instructions.
Direct Inject: Allows direct injection of sample through either the Workstation pump (not available when
Buffer Blending is turned on) or auxiliary load pump. Pre-pump valves (such as the SVT3-2, SV5-4, or
AVR9-8) automate direct injection. Use of an auxiliary pump (such as the Bio-Rad Model EP-1 Econo
pump or Econo Gradient Pump) allows direct injection of sample onto a low pressure column. Use of an
auxiliary (AUX) pump and valve allows multiple direct injections onto a low pressure column. Refer to
Chapter 8 for discussion of a direct inject applications.
• Volume: Allows you to specify the volume of the sample to be injected. The smallest volume selectable
is 0.1 ml; the largest volume selectable is 9999 ml (for larger volumes, use another Load/Inject Sample
step). Actual load volume is defined by the sample loop size.
• Flow Rate: Allows you to specify the flow rate for the sample injection step.
• OK: Adds the step to the protocol. This is the same as pressing the Enter key on the keyboard.
• Cancel: Does not add the step to the protocol. This is the same as pressing the Esc key on the
keyboard.
• Step, Time or Volume: Identifies current step number, and calculates the elapsed time or volume from
all previous steps. This is not user editable.
MODES OF OPERATION SYSTEM OPERATION
7-13