Modes of operation system operation 7-4 – Bio-Rad BioLogic Signal Import Module (SIM) User Manual

Page 133

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7.2

SETUP SCREEN

The Setup screen (see Figure 7-3) is used to select instruments and devices for use in a user-defined
method, name (alias) the Workstation and Maximizer inlets and create Buffer Blending buffer systems (Buffer
Editor). Instruments and devices may include: fraction collector (BioFrac, Model 2128, Model 2110 or generic
(non-Bio-Rad)); detector (Standard UV, QuadTec UV-Vis, pH, Conductivity, SIM); Valves (SVT3-2, SV5-4,
AVR7-3, AVR9-8), auxiliary pump (Econo Gradient, Econo EP-1 or a generic (non-Bio-Rad) pump) and a
Buffer Blender. When a new method is created, the devices defined in the default setup are automatically
loaded into the "Devices in setup" list. For this reason, devices that are used routinely should be saved as
part of the default setup with the File/Save Setup function. Furthermore, the Method Template feature
determines the type of detector (Standard UV or QuadTec) that should be included in each template from
the default setup.

Devices are added to the setup using the "Available Devices" buttons shown in Figure 7-3. When a device is
selected, a dialog is displayed that allows device specific parameters to be entered. When the maximum
allowed number of a device has been added to the "Devices in setup" list, the corresponding "Available
Devices" button becomes grayed out. Devices may be removed from the "Devices in setup" list by pressing
the toolbar Delete button. Double-clicking a device in the "Devices in setup" list opens a dialog used to edit
the device settings.

Figure 7-3. Setup Screen

Devices in setup

UV Detector

Conductivity Monitor

AVR7-3 Valve - Sample Inject

Port 10

Available Devices

Maximizer + Gradient Pump: F10

SVT3-2

Valve

AVR7-3

Valve

SV5-4

Valve

AVR9-8

Valve

Detectors

Fraction

Collector

SV3

INJECT

P

U

M

P

Aux Load

Pump

Inlet A1:

Buffer A1

Inlet A2:

Buffer A2

Inlet B1:

Buffer B1

Inlet B2:

Buffer B2

BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>

Edit

Method

New

Method

New

Run Browser

Manual Setup

Delete

Protocol

wash

load

1

2

Run

Notes

Report

PostRun Log

Settings

WL1 - 280nm

0.40

WL2 - 260nm

0.15

WL3 - 214nm

2.00

WL4 - 405nm

0.00

Flow Rate

EGP %B

0 %B

0.00 ml/min

0%

% Split

1.00ml/min

0 %B2

Maximizer + Gradient Pump: F10

UV

Conductivity

438 psi

1.003 AU

1.23 mS/cm

QuadTec

Econo Gradient

Pump

SIM1/pH

SIM1/SIG

0.548 Volt

7.00 pH

File

View Utilities Options Window Help

Edit

Bio-Rad

Web

Buffer Blender

Is assigned to buffer blender

Is assigned to buffer blender

Is assigned to buffer blender

Is assigned to buffer blender

Buffer Editor...

MODES OF OPERATION

SYSTEM OPERATION

7-4

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