Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 101)

5.0 INTRODUCTION TO THE SYSTEM SOFTWARE
The BioLogic DuoFlow system software is run on computers running the Microsoft Windows ® 2000 operating
system. This chapter discusses the DuoFlow system software version 5.0.
5.1 SYSTEM INTERFACE
The Manual screen (Figure 5-1) is the first screen displayed when the BioLogic software is started. This
screen, like all DuoFlow screens, is grouped into the system menus, the control window, and the status bar.
The use of the Control window functions is discussed in Chapters 6 and 7 and in the separate
documentation for the Econo Gradient Pump (EGP) and the QuadTec UV/VIS detector. Note that many
menus and tool bar functions are grayed out and inacessible until you select, or enter a User name
in the Browser, see chapter 6.
• Browser screen: Chapter 6
• Manual screen: Chapter 7, section 7.1
• Setup screen: Chapter 7, section 7.2
• Protocol screen: Chapter 7, section 7.3
• Run screen: Chapter 7, section 7.4
• Post Run screen: Chapter 7, section 7.5
Figure 5-1. Layout of the Screen Display, showing the Manual Screen
DROP-DOWN
MENUS
CONTROL
WINDOW
MAIN
MENUS
STATUS
BAR
TOOLBAR
CONTROL
PANELS FOR
INSTRUMENTS
CONNECTED TO
INSTRUMENT
BUS
HINT
BOX
WORKSTATION
AND MAXIMIZER
VALVE CONTROL
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
Edit
Method
New
Method
New
Run Browser Manual Setup Setup Protocol
wash load 1 2
Run Notes Report PostRun Log Setup
Bio-Rad
Web
WINDOWS CONTROLS WINDOW TITLE BAR
STATUS BOX
START
Maximizer+Gradient Pump: F-10
pH
Tris (20 mM)
1.00
7.05
0.25 Inlet B
Flowrate ml/min
%
High limit:
0
1000
Low limit: psi.
AVR7-3 at port 4
Workstation Valves
SVT3-2 at port 1 SV5-4 at port 2
P L 2 1
2 1
3 4
STOP START STOP
Set
Set
psi.
Econo Gradient Pump 1
EGP %B
1.00
0
0.00 % Split
Flow
Direction
Flowrate ml/min
%
%
Set
Mode: System Local Mode: System Local Mode: System Local
Lamp Type
Range
Deuterium
190 - 370 nm
nm 280
I
AVR9-8 at port 5
3 7
8 2
6
5
4
I
QuadTec Detector
ON OFF
Zero Baseline
Wavelength Selection
UV Conductivity
Clear
Traces
Resize
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH
CHROMATOGRAM
DISPLAY
CONTROL
Settings
nm 260
nm 214
nm 405
File View Utilities Options Window Help Edit
Advance
Fraction Collector: BioFrac
START STOP
Tube #:
Rack Pos:
Tube #:
A
Tube #: Grid #:
ml
Vol. left:
Mode: System Local
Start:
Rack:
End:
Fraction size:
1 1F
1A
1
90
1.00
A
B
2.00 100% Buffer B
0 2 4 6 8 10
Min. Tenth AU mS/cm
1.50
1.00
0.50
50
0.00
500.0
0.0
400.0
300.0
200.0
100.0
Fractions
Rack: F1 (12-13 mm tubes)
B
INTRODUCTION TO THE SYSTEM SOFTWARE SYSTEM OPERATION
5-1