Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 190)

MULTIPLE COLUMNS ADVANCED SYSTEM APPLICATIONS
The Buffer Editor is used to create new buffer systems or to edit existing buffer systems. Note that Read
Only buffer systems cannot be modified. To modify a Read Only buffer system, uncheck the Read Only box
or Edit a Copy of the buffer system. The following procedure should be used to create or edit a buffer
system.
1. Define a buffer salt
a. From the Buffer Editor main screen press Edit Buffer List
b. Use the Buffer Salt Name list to select and view the desired buffer. If the buffer is in the list, and
it is made from reagents available in your laboratory press Done and go back to the main
screen.
c. If the buffer is in the list but is made of reagents that are not available in your laboratory, press
Copy, rename the buffer and change the buffer information as necessary. Save the buffer, press
Done and go back to the main screen.
d. If the desired buffer is not in the list, press New and enter the required buffer information. Save
the buffer, press Done and go back to the main screen. The "Use Activity Correction" option is
used to correct for the pKa dependence on buffer ionic strength. In practice, this option should
be checked for all pKa's where the charge of one of the associated buffer ions is greater than 0
or less then –1.
2. Define a Salt
a. From the Buffer Editor main screen press Edit Salt List.
b. Use the Salt Name pull-down list to select and view the desired salt. If the salt is in the list and is
made from reagents available in your laboratory press Done and then back to the main screen.
c. If the desired salt is not in the list Copy an existing Salt or Press New to create a new salt.
Enter the required information, save the salt and press Done.
3. Create or Edit a Buffer System
a. From the Buffer Editor main screen press Create New Buffer System to create a new buffer
system or press Edit Current Buffer System to copy and edit an existing "Read Only" buffer
system.
b. From the Buffer selection screen select the number of buffers that will be included in the buffer
system (1, 2 or 3) and then select the buffer(s) and specify their 2X concentration. Press Next
and to to the next step.
c. Select a salt from the salt selection screen and specify its 2X concentration. Press Next and go
to the next step.
d. Select the batch size for the solutions that will be prepared. Note that this will automatically
name each solution and generate the recipe text. Change the solution names and recipe text as
desired and press Next and to go the next step.
e. From the Save Buffer System screen, enter the required information and then press Save. The
following information will be helpful in setting the pH range and reference temperature.
•
pH Range: The "View Table" feature can be used to estimate the buffer pH range. The
usable pH range is generally between 10 % and 90 % A2 but also depends on the ionic
strength (%B) of the buffer. The table will include user-defined pH corrections if you are
editing an existing buffer system that is also currently defined in the method setup and uses
pH corrections. User-defined pH corrections are stored in the method setup and not in the
Buffer Editor.
• Reference Temperature. This is the temperature at which the Buffer Blending tables are
calculated (usually set to 25ºC except as noted below). The Maximizer uses the buffer
temperature coefficient to correct the Buffer Blending tables from the reference temperature
to the run temperature. In the case of multiple component buffers (buffers with more that
one type of buffer), an average temperature coefficient is used to correct the tables. In this
case the Reference Temperature should be set near the run temperature to obtain the best
pH accuracy.