Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 82)

3.6.2 QuadTec UV/Vis Detector
The QuadTec detector is shipped with a “dummy” flow cell installed. Before operating the detector, you need
to install the biocompatible 3mm PEEK flow cell. For complete discussion of the QuadTec detector, including
the procedure for installing the flow cell, refer to the QuadTec Instruction Manual.
1. Make sure the detector power is OFF.
2. Loosen the two knurled screws on the front of the flow cell and gently pull them out. This allows the
flow cell housing to slide out.
3. Remove the dummy cell by gently pulling it upward. Save the dummy cell in a secure place. It is
required for measuring signal and reference output values for the lamps. See Section 5.1, Checking
the Status of D2 Lamp, in the QuadTec instruction manual for instruction on recording signal and
reference values.
4. Insert the new flow cell and make sure that the engraved specifications point towards the user and
that the fixing hole on the back side of the cell meets the corresponding metal pin of the detector's
housing.
5. Slide the complete system towards the detector, insert the two knurled screws, and tighten them by
hand.
6. Attach the 10-32 Fingertight fittings and tubing to the inlet and outlet ports and use a syringe to rinse
the flow cell with 10-20 ml of 100% analytical grade methanol, followed by 5 ml distilled deionizied
(DDI) water.
7. Connect the QuadTec to the DuoFlow system. If the Maximizer is to be used, the QuadTec should
be connected to the Maximizer rear panel Com 1 connector using System Cable 25. If the
Maximizer is not in use, connect the QuadTec via the Instrument Control Module (ICM), as
discussed on page 3-11.
Figure 3-9. QuadTec Detector
3
Link
Load
Prog
Hold 0
Run 1
Time
(min)
1
(nm)
2
(nm)
Edit Prog
Clear O
Eve
nts: 2 off 0 o
n / Signal 1
[ au ]
Signal 2
[ au ]
Vie w
Setup
GLP
AUT
O
ZER
O
SCAN
1
2
3
4
5 6
7
8
9
0
SLIDES
FLOW CELL
FLOW CELL HOUSING
FLOW PATH PORT
FLOW PATH PORT
0
SYSTEM SETUP SYSTEM INSTALLATION AND SETUP
3-10