Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 193)

MULTIPLE COLUMNS ADVANCED SYSTEM APPLICATIONS
10-6
6. Measure the pH of the low salt and then the high salt solutions using a high quality pH probe. Be
sure to correct the reading for any difference between the sample temperature and run temperature.
Note that if you are using a multi-component buffer system and want to use a buffer specific pH
correction you will need to repeat steps 2 through 6 for each buffer component.
7. Load your experimental method from the Browser dialog and press Setup.
8. Double click on the Buffer Blending icon, and select the desired buffer. The appearance of the Buffer
Blending setup dialog depends on whether a single component (see Figure 10-1) or multiple
component buffer is selected (see Figure 10-2).
9. Place a check in the pH correction box and select the Two-Point Correction option.
10. Enter the desired pH in the Desired pH box.
11. Enter the low salt and high salt pH's that were measured in Step 6 in the Observed pH box and then
enter the %B that was used to collect the high salt pH. For multi-component buffers repeat this for
each buffer in the buffer system. The software will calculate the correction.
(Optional) Check the pH Correction
12. From the manual screen press the Buffer Blender "Setup" button and select the desired Buffer
System from the recipe list. Turn pH correction on and enter the Desired and Observed pH from
step 10 above.
13. Set the pH, Salt composition and flow rate. Press run.
14. After the system has equilibrated collect some effluent and measure the pH using a high quality pH
probe.