Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 165)

Figure 7-8. Protocol Screen during a Run
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Mode
Edit View Utilities Options
Edit
Method
New
Method
New
Run Manual Setup Protocol
wash
load
1
2
Run Notes Report PostRun Log
Help
1
2
3
4
5
0.0
0.0
2.0
3.0
Collect Fractions during entire method
Volume Description Parameters
Chart Recorder
Isocratic Flow
25mM Tris
25mM Tris + Salt 100%
0%
7.0
Time: 2.0 min
Flow: 2.0 ml/min
Turn ON
Linear Gradient
100%
0%
Volume: 4.0 min
Flow: 2.0 ml/min
6
100% --> 55%
0% --> 45%
Volume: 16.0 min
Flow: 2.0 ml/min
7
23.0
Isocratic Flow
55% --> 100%
45% --> 0%
Volume: 2.0 min
Flow: 2.0 ml/min
25.0 Linear Gradient
8
100%
0%
Volume: 3.0 min
Flow: 2.0 ml/min
28.0
29.10
Isocratic Flow
Load: Sample
End of Protocol
Static Loop
Load: standard
Volume: 1.0 ml
Auto Inject Valve
Flow: 2.0 ml/min
25mM Tris
25mM Tris + Salt
25mM Tris
25mM Tris + Salt
25mM Tris
25mM Tris + Salt
25mM Tris
25mM Tris + Salt
File
Browser
Window
BioLogic Duo-Flow - offline
Settings
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH
Bio-Rad
Web
Fraction
Collection
Edit Method
wash
load
1
2
hold
alarm
3
4
MODES OF OPERATION SYSTEM OPERATION
7-36