Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 174)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
8-1
8.0 SAMPLE LOADING
The BioLogic DuoFlow system is capable of running both simple and complex experiments types. This
Section describes how the optional hardware components can be used to incorporate a variety of advanced
features into a method.
The BioLogic DuoFlow system supports several methods for sample loading. Typically a sample is loaded
through a static fixed volume loop as described in Chapter 4. However, when automation or large volume
sample injection is required, the following options are available:
• Automatic loop fill and rinse using an auxiliary pump inlet valve and an auxiliary pump
• Direct sample loading onto low-pressure columns using an auxiliary pump such as the Econo
Gradient Pump (EGP) or EP-1 Econo pump.
• Direct sample loading through the Workstation pump
• Sample loading using the DynaLoop sliding-piston loop
8.1 AUTOMATIC LOOP FILL AND RINSE
With the addition of an Econo Gradient Pump, EP-1 Econo pump or other compatible pump as an auxiliary
load pump along with an auxiliary pump inlet valve (AVR9-8), the system can load up to 7 samples
sequentially into a loop. One of the Aux pump inlet valve ports must be assigned to a rinse solution.
Similarly, an SVT3-2 or SV5-4 Aux pump inlet valve may be used to load 1 samples or 3 sample,
respectively.
System Setup
1. Connect an auxiliary pump to the system as described in Section 3.9.1 (EP-1 or non-Bio-Rad pump)
or 3.9.2 (Econo Gradient Pump).
2. Plumb the Aux Pump Inlet valve and Aux Pump to port 2 of the AVR7-3 Sample Inject valve as
shown in Figure 8-1.
3. In the device setup screen, add an Aux Pump to the setup and define it as a load pump. Define
non-Bio-Rad pumps as an EP-1 load pump.
4. In the device setup screen, add an AVR9-8 (or SV5-4 or SVT3-2) valve. Define the valve as an Aux
Pump Inlet valve and name each position. One position (usually position 1) should be defined as a
"Rinse" solution. The rinse solution is used to clean the sample loop and sample inlet lines between
injections. Note that some sample will be lost in the process of pulling sample from a remote beaker.
Be sure to factor in the solution volume required to fill all the buffer lines leading up to the top of the
column when programming the injection step.
5. When using a low-pressure column, such as an Econo-Pac cartridge, remove the 40-psi
backpressure regulator from the post-column position and place it between the Workstation pump
and the mixer.