Bio-Rad BioLogic Signal Import Module (SIM) User Manual

Page 52

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The Model 2128 is controlled by the DuoFlow Controller via the Instrument Bus. See Chapter 3.8.3 for
connection instructions.

The Model 2128 is plumbed to the DuoFlow system using 1/16” (1.6 mm) OD inlet tubing and either of the
following fittings:

To connect directly to the dispenser head, use 1/4-28 flat bottom fittings.

To connect to the optional on-arm diverter valve, use the 10-32 nut and ferrule fittings supplied with
the valve.

When the Model 2128 is configured in the Setup screen, collection schemes such as Collect All, Threshold,
Collection Windows, and Threshold + Collection Windows (all with Delay volume, if required) automatically
become available in the Protocol screen. The starting and ending tube numbers also may be specified. The
Model 2128’s optional diverter valve mounts on the fraction collector’s arm and minimizes liquid spills during
fraction advances. If this valve is used, it is automatically sensed by the system and hence is not configured
by the user in the Setup screen.

This instrument is described in detail in its separate documentation.

2.7.4 Generic Fraction Collectors

A non-Bio-Rad collector may be used as an integral part of the DuoFlow system providing its tube advance
function can be initiated by an active low TTL signal (>100 ms). Electrical connection from the collector is to
pin 5 (Frac Advance) and pin 9 (ground) of the Workstation’s AUX connector. See the pin-out information in
your collector manual.

With an optional SVT3-2 diverter valve configured as a fraction collector diverter valve in the Setup screen,
the DuoFlow Controller will control all collection parameters including advanced features such as collection
by Threshold and/or Collection Windows. If the optional valve is not used, then only Collect All is available.

Fraction advance marks to a Bio-Rad chart recorder are embedded on the UV signal from the Workstation.

DESCRIPTION OF SYSTEM COMPONENTS

SYSTEM OVERVIEW

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