Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 183)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
9-2
Writing the Column Switching Protocol
When using multiple columns, care should be taken to prevent cross-contamination. Purge steps should be
included before placing a column inline to ensure that the tubing is clean and filled with the appropriate
buffer. The following three steps should be included as part of a column-switching step.
1. Add a Change Valve step to your protocol and place the Column Switcher valve in the Purge
position.
2. Add an isocratic flow step to wash the tubing and mixer with 3-5 volumes of buffer.
3. Add a Change Valve step to your protocol and select the desired column on the Column Switcher
valve.
Column-switching steps are usually placed at the beginning of a protocol or at the start of each dimension in
a multi-dimensional chromatography experiment.
9.1.2 AVR9-8 Eight Column Switching
Figure 9-2. Column Switching using Two AVR9-8 Valves
For this application, two AVR9-8 valves are required.
UV DETECTOR AND
FRACTION COLLECTOR
AVR9-8 AS COLUMN
SWITCHING VALVE
TO RECEIVE SAMPLE
AVR7-3 INJECT VALVE
FOR SAMPLE APPLICATION
COLUMN INLET
SECOND AVR9-8 AS
COLUMN SWITCHING VALVE
TO DIRECT ELUENT,
TO DETECTORS AND
FRACTION COLLECTOR
COLUMN OUTLET
5
4
3
2 1
7
6
9
UP TO 8
DIFFERENT
COLUMNS
8
5
4
3
2 1
7
6
9
8
COLUMN
WORKSTATION
PUMP
SAMPLE
LOOP
SAMPLE
INJECT
WASTE
WASTE
6
7
1
2 3
4
5
INJECT