Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 150)

Table 7-10. (continued)
Fraction Collection
Description: Threshold
Threshold: This method allows the flow to be sent to the collector if the detector signal of the eluant is
above or below a certain signal level. Note: You can select any detection source to initiate threshold-based
fraction collection. Non-peak eluant can be sent to waste or to the fraction collector.
• Fraction Size, Start Tube, End Tube, Start Rack and End Rack: See description for Collect All on
page 7-20.
• Threshold: Enter the threshold value. Fractions will be collected whenever the detector output goes
above or below the threshold level.
• Above/below Threshold: Used to select whether fractions are collected when the detector signal is
above or below a designated Threshold.
• Non-peak parameters Destination and Fraction Size: Enter the non-peak parameters. If the buffer
stream is diverted to Waste, there is no need to enter a fraction size. But if you want to collect non-peak
material, you must enter a fraction size.
Collect All
Threshold
Collection Windows
Threshold and Collection Windows
Fraction Collection Scheme
BioFrac Rack Type: F1 (12-13 mm tubes)
Close
Waste
Destination:
Non-peak Parameters:
Tubes
Below Threshold
Above Threshold
A
B 90
Rack:
1
1A
1F
Tube #: Row Col.
Start:
End:
1.00
Fraction Size
ml
0.100
Threshold
1.00
Fraction Size
ml
UV Detector
Threshold Detector
AU
MODES OF OPERATION SYSTEM OPERATION
7-21