Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 119)

6.2 METHOD TEMPLATES
The BioLogic software includes Method Templates to simplify the method creation process. These templates
can be used as is, or modified to fit experimental requirements. The templates that are available depend on
the system configuration (BioLogic DuoFlow, BioLogic DuoFlow QuadTec, BioLogic DuoFlow Maximizer and
BioLogic DuoFlow Pathfinder systems). Before using the Method Template feature, the system must be
configured in the BioLogic Configuration utility and a QuadTec or UV detector should be defined in the
default setup (see Section 7.2).
Figure 6-5. New Method Dialog Showing Method Templates.
To load a Method Template:
1. Select New/ New Method from the Browser toolbar or the New Method button on the system tool
bar.
2. In the New Method dialog place a check in the "Use Method Templates" box (see Figure 6-5).
3. Select the Experiment Type
4. Select the Method Template. A brief description of each method template is given in the "Template
Description" box, or a more detailed description can be viewed from the online help by pressing
"View Template Help".
5. After entering the method name press OK to load the template.
6. If your system includes a Model 2128 fraction collector you will need to change the Method Template
Setup. To this, go to the Setup Screen and delete the BioFrac fraction collector and replace it with a
Model 2128. In the protocol screen open the fraction collection step and make sure the correct
number of tubes is defined.
New Method
User Name:
Project Name:
Method Name:
Method Description:
Method Author:
Method Identification
The select method template
will be used to create the method.
Template Selection
Experiment Type:
Affinity
Chromatofocusing
Hydrophobic Interaction
Hydroxyapatite
Ion Exchange
Gel Filtration
METHOD DESCRIPTION: Size Exclusion with Auto Inject (C).
This Method is used to separate molecules on the basis of molecular size. Loaded samples are
injected automatically with an AVR7-3 sample inject valve.
COLUMN/SAMPLE: This method was written for a 490 to 530 ml gel filtration column (2.5 x 100 cm
to 2.6 x 100 cm) but can be scaled to work with columns of different sizes. Sample volumes should
typically be 1 to 3% of the column volume. Consult your column instructions for specific sample
size and flow rate information.
REQUIRED DEVICES: BioFrac fraction collector, AVR7-3 sample inject valve, conductivity
monitor, standard UV detector and a sample loop (up to 20 ml).
METHOD PARAMETERS
Flow Rate 2.5 ml/min
Sample Size + Rinse: 20 ml
Fraction Size 2.5 ml
Fraction Collector Rack: F1 (12-13 mm tubes) Size Exclusion with Auto Inject (A)
Size Exclusion with Auto Inject (B)
Size Exclusion with Auto Inject (C)
Size Exclusion with Auto Loop Fill _Rinse (A)
Size Exclusion with Auto Loop Fill _Rinse (B)
Size Exclusion with Auto Loop Fill _Rinse (C)
Use Method Templates
Phoenix
Project A1
Ion Exchange with Auto-Inject
James R.
Cancel
View Template Help
OK
Template Description:
Templates:
INTRODUCTION TO THE BROWSER SCREEN SYSTEM OPERATION
6-7