Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 177)

SAMPLE LOADING ADVANCED SYSTEM APPLICATIONS
8-4
When doing Aux Pump Direct Injection the AVR7-3 injection valve operates as follows:
• Load: In this position, the valve connects ports 4 and 5 and is used for column equilibration and
sample elution.
• Inject: In this position, the valve connects ports 3 and 4 and is used to inject sample onto the
column.
• Purge: In this position, the valve connects ports 5 and 7 and is used to take the column out of line
for purging or buffer changes.
Figure 8-3. AVR7-3 Valve Positions During a Run with Direct Sample Loading and Injection
8.3 GRADIENT PUMP DIRECT INJECTION
Large volume samples can be directly loaded through the Workstation pumps. Up to 7 samples can be
sequentially loaded if an inlet valve (AVR9-8) is added to the setup. One of the inlet valve ports must be
assigned to the running buffer. Similarly, an SVT3-2 or SV5-4 Aux pump inlet valve may be used to load 1
sample or 3 samples, respectively. Samples loaded through the pumps should be filtered through a 0.45 um
filter. Furthermore, the pump should be washed with a sanitization solution afterwards to remove residual
protein contamination that could reduce the piston seal lifetime. Consult the DuoFlow online help for
additional information.
System Setup
1. Connect a valve (SVT3-2, SV5-4 or AVR9-8) to Inlet A or B as shown in Figure 8-4. For systems
configured with a Maximizer, valves can be connected at Inlets A1, A2, B1 and B2.
2. In the device setup screen, define a connected valve (SVT3-2, SV5-4 or AVR9-8) as an Inlet A (or
Inlet B) valve and name each valve position. As shown in Figure 8-4, one position (usually position
1) should be defined as the running buffer. To automate system cleaning, ports may also be
assigned to sanitization and storage solutions.
3. To prevent air from entering the system, unused valve ports should be plugged with the ¼-28 plugs
supplied in the DuoFlow fittings kit.
LOAD INJECT PURGE
LOW
PRESSURE
COLUMN
AUXILIARY
PUMP
PURGE
AUXILIARY
LOAD PUMP
WORKSTATION
PUMP
WASTE
WASTE
6
7
1
2
3
4
5
LOW
PRESSURE
COLUMN
AUXILIARY
PUMP
PURGE
AUXILIARY
LOAD PUMP
WORKSTATION
PUMP
WASTE
WASTE
6
7
1
2
3
4
5
LOW
PRESSURE
COLUMN
AUXILIARY
PUMP
PURGE
AUXILIARY
LOAD PUMP
WORKSTATION
PUMP
WASTE
WASTE
6
7
1
2
3
4
5