Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 132)

Maximizer + Gradient Pump: This panel is displayed in place of the Gradient Pump panel when a
Maximizer is connected to the system and is used to control buffer composition, flow rate and pressure limits
(see Table 2-3) as well as to start and stop the pumps. When Buffer Blending is turned on, composition is
determined by pH and % Inlet B. When Buffer Blending is turned off, composition is determined by percent
Inlet A1 or A2 and percent Inlet B1 or B2. The Start/Stop buttons are used to start and stop the pumps. The
Set button is used change the composition, flow rate and pressure limits while the pumps are running. The
System and Local option buttons are used to toggle the Maximizer between System and Local mode.
Fraction Collector: This panel is used to control a fraction collector. Its appearance depends on the type of
fraction collector connected to the system (BioFrac or Model 2128). From this panel the Start Tube, End
Tube, Fraction size and rack type are set and the fraction collector can be started or stopped. Also displayed
on this panel are the current "Tube number" and the tube "Volume left" to fill. The System / Local option
buttons are used to toggle a BioFrac or Model 2128 fraction collector between System and Local mode. Only
fraction collection by volume is supported in manual mode. When a BioFrac is connected to the system the
rack grid numbers for the start and end tubes are also displayed.
UV Detector / Chart Recorder / Signal Import Module: This panel is used to control the standard UV
monitor and chart recorder as well as to monitor the status of the two signal import modules (SIM 1 and
SIM 2), if they are connected to the they system (see Chapter 2.9.6 for more information). The UV detector
panel is used to turn the Standard UV detector lamp on and off and to zero the baseline. The Chart
Recorder panel is used to start/stop the chart recorder and to set the conductivity and UV range. Event
marks can be added to the chart recorder output by pressing the "Event mark" button. The down arrow in the
upper right corner of the panel is used to toggle between the QuadTec and UV detector control panels.
QuadTec Detector: This Panel is used to control the QuadTec detector. From this panel, the lamp type and
wavelength range is viewed, the QuadTec detector can be turned on or off, up to four wavelengths can be
selected and the UV-Vis baseline can be zeroed. For best performance, the QuadTec wavelengths should be
entered in numerical order, starting in the upper most field. The Set button is used to accept any changes
made in the Wavelength Selection boxes. The down arrow in the upper right corner of the panel is used to
toggle between the QuadTec and UV detector control panels. The System / Local option buttons are used to
toggle the QuadTec between System and Local mode.
Econo Gradient Pump: This panel allows you to control an Econo Gradient Pump (EGP) if it is connected
to the system bit bus. From this panel, you can control the flow rate, % split (see Chapter 2.8.3) and flow
direction as well as start and stop the pump. For more detail see the EGP instruction manual.
Workstation Valves / Maximizer Valves: These two panels are used to control any of the solenoid and
automated valves connected to the workstation and Maximizer. The down arrow in the upper right corner of
the panel is used to toggle between the workstation and Maximizer valves.
Chromatogram: This panel is used to view up to 8 instrument traces during a manual run. Traces may
include: UV detector, conductivity, pH, pressure, theoretical %B, four QuadTec wavelengths, and signals from
two external detectors (see Chapter 2.9.6 and Table 2-7 for more information about the SIM-HR and the
Maximizer SIM, respectively). The chromatogram display is controlled through a combination of the
Chromatogram Settings dialog (Settings button or Options pull-down menu), the Resize and Clear Traces
buttons and the scroll bars. Each scroll bar is assigned to an individual trace by the drop-down menu above
it.
Status Bar: This panel is used to display numerical data from the BioLogic DuoFlow workstation and its
attached devices. Parameters include: gradient pump flow rate, %B and pressure; UV detector absorbance,
QuadTec UV-Vis wavelength and absorbance; conductivity; SIM data (from a pH probe or an external
detector) as well as the Econo Gradient Pump flow rate, %B and % Split.
MODES OF OPERATION SYSTEM OPERATION
7-3