Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 160)

Figure 7-5b. Run Screen showing a Run in Progress with a Time-based Chromatogram
and "Rack and Grid#" fraction numbering
Advance
Frac. Collector
Divert Valve
UV Detector
Set
UV Range
Zero
Baseline
Event
mark
Cond Range
500
Protocol: > 1 0:00.0 Collection Fractions of size 2.00 ml during entire run
Scout: Method 7 - Scout Run - [S] Scout Buffer A A1 [ 1 of 1 ] > [S] Scout Buffer A A1
Collect
Waste
Grad. Pump
Chart Recorder
1000
High psi
0
Low psi
QuadTec
Zero
Baseline
0.01
Conductivity UV
Pause Abort Hold Full View
PULL DOWN MENU
TO SELECT LEFT TRACE PULL DOWN MENU
TO SELECT RIGHT TRACE
ACTIVE ACTIVE
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
File View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup Protocol
wash
load
1
2
Run Notes Report PostRun Log
Window Help
Settings
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
Run Time
0:00.0
Run Volume
0.0 ml
Step Time Left
0:00.0
Fraction Vol. Left
0.0 ml
WL1 - 280nm
Port 2: User Valve; Position 1
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH
Edit
Bio-Rad
Web
100.0% Buffer B
50.0
0.00
1F
A
2F 3F 4F 5F 6F 7F 8F 9F 10F 11F 12F 13F14F
4.00 2.00 6.00
AU mS/cm
0.0
50.0
40.0
30.0
20.0
10.0
-10.0
0.100
0.075
0.050
0.025
-0.000
Min. Tenth
Fractions
Rack Pos:
Grid #:
B A
MODES OF OPERATION SYSTEM OPERATION
7-31