Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 164)

7.4.3 Editing A Method During a Run
During a run, you may find you want to change some of the run parameters. The figure below shows how
this is handled by the system and some of the restrictions that apply.
Figure 7-7. Editing during a Run
• During the first run of a method, you can pause the method and return to the Protocol screen to edit
steps that have not already been started. The method name will not change.
• Any run after the first can be paused and then edited, except runs in queue or scout which cannot
be edited. You will first be asked to rename the method (or accept the default new name) when the
Edit Protocol button is clicked. This is to ensure the integrity of the database in terms of which
method protocol a particular run is tied to.
For example, assume you make three runs under a method named “Ion-exchange UNO Q1.” During
the fourth run, you may decide to pause the run, and enter the Protocol screen to increase the
length of the salt gradient. In this case, you will first be prompted to rename the method (e.g.,
Ion-exchange UNO Q1 Rev. 1) before proceeding with the edit.
Upon completion of the run, the database of methods and runs will show three runs associated with
the method “Ion-exchange UNO Q1” and one run associated with the method “Ion-exchange UNO
Q1 Rev. 1.”
• A fraction collection scheme is editable, but it cannot be added to a protocol once a run has been
started. In such cases, you should abort the run and select Edit the Method.
• A currently programmed fraction collection scheme may be edited during a run, but note that
changing the fraction size of a “Collect All” scheme will first require selection of “Collection Windows.”
This is because the editing process effectively turns the initial “Collect All” scheme into the “first”
collection window.
Method
Edit prior
to run
Edit during or after any run after the first run
Edit during
the first run
Setup
Protocol
(Steps)
Method
Name
Run 1
Run 2
Run 3
MODES OF OPERATION SYSTEM OPERATION
7-35